CLARITY tissue-clearing method

E47391

imaging preparation method neuroscience technique tissue clearing technique

CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.

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Observed surface forms (1)

Surface form	Occurrences
Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel	1

Statements (50)

Predicate	Object
instanceOf	imaging preparation method ⓘ neuroscience technique ⓘ tissue clearing technique ⓘ
acronym	CLARITY ⓘ
advantage	avoids physical sectioning of tissue ⓘ maintains spatial relationships in intact organs ⓘ
appliedTo	human brain tissue ⓘ mouse brain ⓘ other organs ⓘ spinal cord ⓘ
compatibleWith	confocal microscopy ⓘ fluorescent proteins ⓘ immunohistochemistry ⓘ in situ hybridization ⓘ light-sheet microscopy ⓘ two-photon microscopy ⓘ
developedAt	Stanford University ⓘ
developedBy	Karl Deisseroth ⓘ Kwanghun Chung ⓘ
enables	deep tissue imaging without sectioning ⓘ high-resolution imaging of brain tissue ⓘ three-dimensional imaging of intact organs ⓘ
field	connectomics ⓘ histology ⓘ neuroanatomy ⓘ neuroscience ⓘ
fullName	CLARITY tissue-clearing method self-linksurface differs ⓘ surface form: Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel
inspired	development of PACT tissue clearing ⓘ development of PARS tissue clearing ⓘ development of other hydrogel-based clearing methods ⓘ
keyStep	electrophoretic tissue clearing ⓘ hydrogel embedding of tissue ⓘ lipid removal ⓘ passive tissue clearing ⓘ
limitation	clearing process can be time-consuming ⓘ requires specialized equipment for active clearing ⓘ
preserves	fine structural details ⓘ nucleic acids ⓘ proteins ⓘ
publicationYear	2013 ⓘ
relatedTo	3DISCO tissue clearing method ⓘ Scale tissue clearing method ⓘ SeeDB tissue clearing method ⓘ
renders	biological tissues optically transparent ⓘ
usedFor	mapping neural circuits ⓘ pathological analysis of brain tissue ⓘ studying brain connectivity ⓘ visualizing long-range axonal projections ⓘ
uses	acrylamide-based hydrogel ⓘ sodium dodecyl sulfate detergent ⓘ

Referenced by (2)

Full triples — surface form annotated when it differs from this entity's canonical label.

CLARITY tissue-clearing method → fullName → CLARITY tissue-clearing method self-linksurface differs ⓘ

this entity surface form: Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel

Karl Deisseroth → knownFor → CLARITY tissue-clearing method ⓘ