Scale tissue clearing method
E261426
The Scale tissue clearing method is a chemical technique that renders biological tissues optically transparent while preserving fluorescent signals, enabling deep, high-resolution imaging of intact specimens.
All labels observed (1)
| Label | Occurrences |
|---|---|
| Scale tissue clearing method canonical | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T2366517 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: Scale tissue clearing method Context triple: [CLARITY tissue-clearing method, relatedTo, Scale tissue clearing method]
-
A.
CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
-
B.
Yale Center for High Throughput Cell Biology
The Yale Center for High Throughput Cell Biology is a research center specializing in large-scale, automated cellular analysis and imaging to advance biomedical discovery.
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C.
Tissues and Issues
Tissues and Issues is a pop-oriented studio album by Welsh singer Charlotte Church that marked her transition from classical crossover to mainstream pop music.
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D.
ImageWorks: The What-If Labs
ImageWorks: The What-If Labs is an interactive, hands-on exhibit at Epcot where guests can explore creativity and sensory illusions through playful, imaginative experiments.
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E.
Interface Clear
Interface Clear is a company specializing in building automation and control systems, particularly focused on integrating and managing HVAC and related building technologies.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: Scale tissue clearing method Target entity description: The Scale tissue clearing method is a chemical technique that renders biological tissues optically transparent while preserving fluorescent signals, enabling deep, high-resolution imaging of intact specimens.
-
A.
CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
-
B.
Yale Center for High Throughput Cell Biology
The Yale Center for High Throughput Cell Biology is a research center specializing in large-scale, automated cellular analysis and imaging to advance biomedical discovery.
-
C.
Tissues and Issues
Tissues and Issues is a pop-oriented studio album by Welsh singer Charlotte Church that marked her transition from classical crossover to mainstream pop music.
-
D.
ImageWorks: The What-If Labs
ImageWorks: The What-If Labs is an interactive, hands-on exhibit at Epcot where guests can explore creativity and sensory illusions through playful, imaginative experiments.
-
E.
Interface Clear
Interface Clear is a company specializing in building automation and control systems, particularly focused on integrating and managing HVAC and related building technologies.
- F. None of above. chosen
Statements (45)
| Predicate | Object |
|---|---|
| instanceOf |
chemical technique
ⓘ
optical clearing method ⓘ tissue clearing method ⓘ |
| aimsTo |
maintain fluorescence
ⓘ
match refractive index within tissue ⓘ reduce light scattering ⓘ |
| allows |
3D reconstruction of neural circuits
ⓘ
whole-organ imaging ⓘ |
| appliedTo |
brain tissue
ⓘ
fixed tissues ⓘ mouse brain ⓘ |
| basedOn | aqueous chemical solution ⓘ |
| category | sample preparation technique ⓘ |
| comparedWith |
CLARITY
ⓘ
CUBIC ⓘ SeeDB ⓘ |
| compatibleWith |
confocal microscopy
ⓘ
light-sheet fluorescence microscopy ⓘ two-photon microscopy ⓘ |
| designedFor | intact tissue imaging ⓘ |
| developedBy | Hiroki Hama ⓘ |
| developedIn | Japan ⓘ |
| effectOnTissue | swelling of tissue ⓘ |
| enables |
deep imaging of intact specimens
ⓘ
high-resolution imaging of thick tissues ⓘ optical transparency of biological tissues ⓘ |
| field |
biological imaging
ⓘ
microscopy ⓘ neuroscience ⓘ |
| firstDescribedIn | 2011 ⓘ |
| firstPublishedIn | Nature Neuroscience ⓘ |
| notableVariant |
ScaleA2
ⓘ
ScaleS ⓘ ScaleU2 ⓘ |
| optimizationGoal | balance between transparency and fluorescence preservation ⓘ |
| preserves |
fluorescent proteins
ⓘ
fluorescent signals ⓘ |
| publicationType | peer-reviewed article ⓘ |
| requires | long incubation times ⓘ |
| usedIn |
connectomics
ⓘ
developmental biology ⓘ Neuroscience ⓘ
surface form:
systems neuroscience
|
| usesComponent |
Triton X-100
ⓘ
glycerol ⓘ urea ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: Scale tissue clearing method Description of subject: The Scale tissue clearing method is a chemical technique that renders biological tissues optically transparent while preserving fluorescent signals, enabling deep, high-resolution imaging of intact specimens.
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.