SeeDB
E902753
SeeDB is a tissue-clearing method that uses high-concentration fructose solutions to render biological samples optically transparent while preserving their structural integrity for deep imaging.
Statements (45)
| Predicate | Object |
|---|---|
| instanceOf |
optical clearing technique
ⓘ
tissue-clearing method ⓘ |
| advantageOverSolventMethods |
better preservation of fluorescence
ⓘ
reduced tissue shrinkage ⓘ |
| aimsTo |
enable deep imaging
ⓘ
render biological samples optically transparent ⓘ |
| appliesTo |
brain tissue
ⓘ
fixed tissue samples ⓘ mouse brain ⓘ |
| clearingMechanism |
reduction of light scattering
ⓘ
refractive index matching ⓘ |
| compatibleWith |
confocal microscopy
ⓘ
light-sheet fluorescence microscopy ⓘ two-photon microscopy ⓘ |
| developedBy | Takeshi Ke and colleagues ⓘ |
| doesNotUse |
detergent-based lipid removal
ⓘ
organic solvents ⓘ |
| field |
bioimaging
ⓘ
neuroscience ⓘ tissue engineering ⓘ |
| finalRefractiveIndex | approximately 1.49 ⓘ |
| hasProperty |
aqueous
ⓘ
high refractive index ⓘ mild clearing conditions ⓘ preserves fluorescence signals ⓘ preserves tissue morphology ⓘ |
| hasVariant | SeeDB2 NERFINISHED ⓘ |
| optimizedFor |
maintaining sample size
ⓘ
minimizing tissue deformation ⓘ |
| preserves |
fine neural structures
ⓘ
structural integrity of tissue ⓘ |
| publicationYear | 2013 ⓘ |
| publishedIn | Nature Neuroscience NERFINISHED ⓘ |
| relatedTo |
CLARITY
NERFINISHED
ⓘ
CUBIC NERFINISHED ⓘ Scale ⓘ |
| requires | stepwise increase of fructose concentration ⓘ |
| samplePreparation | fixed tissue ⓘ |
| speciesPrimarilyTestedOn | mouse ⓘ |
| usedFor |
3D reconstruction of neural circuits
ⓘ
visualization of fluorescently labeled cells ⓘ whole-brain imaging ⓘ |
| usesReagent |
high-concentration fructose solution
ⓘ
thioglycerol ⓘ water ⓘ |
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.