3DISCO tissue clearing method
E261427
The 3DISCO tissue clearing method is a solvent-based technique that renders biological tissues transparent to enable high-resolution three-dimensional imaging of intact organs and organisms.
All labels observed (3)
| Label | Occurrences |
|---|---|
| 3DISCO tissue clearing method canonical | 1 |
| iDISCO tissue clearing method | 1 |
| uDISCO tissue clearing method | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T2366518 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: 3DISCO tissue clearing method Context triple: [CLARITY tissue-clearing method, relatedTo, 3DISCO tissue clearing method]
-
A.
CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
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B.
Light-Space Modulator
Light-Space Modulator is an experimental kinetic sculpture by László Moholy-Nagy that explores movement, light, and shadow through rotating metal and glass forms.
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C.
Biomedical Optics Express
Biomedical Optics Express is a peer-reviewed scientific journal focusing on research and developments in the field of biomedical optics and photonics.
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D.
ImageWorks: The What-If Labs
ImageWorks: The What-If Labs is an interactive, hands-on exhibit at Epcot where guests can explore creativity and sensory illusions through playful, imaginative experiments.
-
E.
The O2 complex
The O2 complex is a large entertainment district in London featuring the O2 Arena, cinemas, restaurants, bars, and various leisure and retail facilities housed under the iconic dome structure.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: 3DISCO tissue clearing method Target entity description: The 3DISCO tissue clearing method is a solvent-based technique that renders biological tissues transparent to enable high-resolution three-dimensional imaging of intact organs and organisms.
-
A.
CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
-
B.
Light-Space Modulator
Light-Space Modulator is an experimental kinetic sculpture by László Moholy-Nagy that explores movement, light, and shadow through rotating metal and glass forms.
-
C.
Biomedical Optics Express
Biomedical Optics Express is a peer-reviewed scientific journal focusing on research and developments in the field of biomedical optics and photonics.
-
D.
ImageWorks: The What-If Labs
ImageWorks: The What-If Labs is an interactive, hands-on exhibit at Epcot where guests can explore creativity and sensory illusions through playful, imaginative experiments.
-
E.
The O2 complex
The O2 complex is a large entertainment district in London featuring the O2 Arena, cinemas, restaurants, bars, and various leisure and retail facilities housed under the iconic dome structure.
- F. None of above. chosen
Statements (39)
| Predicate | Object |
|---|---|
| instanceOf |
solvent-based tissue clearing technique
ⓘ
tissue clearing method ⓘ |
| appliedTo |
central nervous system tissues
ⓘ
embryos ⓘ mouse organs ⓘ |
| basedOn |
lipid removal
ⓘ
organic solvent dehydration ⓘ refractive index matching ⓘ |
| compatibleWith |
confocal microscopy
ⓘ
light-sheet fluorescence microscopy ⓘ |
| developedFor |
whole-body imaging in small animals
ⓘ
whole-organ imaging ⓘ |
| developedIn | early 2010s ⓘ |
| effectOnTissue |
increased optical transparency
ⓘ
volume reduction ⓘ |
| enables | high-resolution 3D imaging ⓘ |
| field |
biological imaging
ⓘ
developmental biology ⓘ neuroanatomy ⓘ |
| hasAdvantage |
high optical transparency
ⓘ
rapid clearing of large samples ⓘ |
| hasLimitation |
quenching of endogenous fluorescence
ⓘ
tissue shrinkage ⓘ |
| optimizedFor | immunolabeled samples ⓘ |
| relatedTo |
CLARITY tissue-clearing method
ⓘ
surface form:
CLARITY tissue clearing method
3DISCO tissue clearing method self-linksurface differs ⓘ
surface form:
iDISCO tissue clearing method
3DISCO tissue clearing method self-linksurface differs ⓘ
surface form:
uDISCO tissue clearing method
|
| requiresStep |
graded dehydration in organic solvents
ⓘ
immersion in high refractive index medium ⓘ lipid extraction ⓘ |
| usedFor |
rendering biological tissues transparent
ⓘ
three-dimensional imaging of intact organisms ⓘ three-dimensional imaging of intact organs ⓘ |
| usedIn |
organ development studies
ⓘ
vascular network imaging ⓘ whole-brain mapping studies ⓘ |
| usesReagent |
dibenzyl ether
ⓘ
dichloromethane ⓘ tetrahydrofuran ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: 3DISCO tissue clearing method Description of subject: The 3DISCO tissue clearing method is a solvent-based technique that renders biological tissues transparent to enable high-resolution three-dimensional imaging of intact organs and organisms.
Referenced by (3)
Full triples — surface form annotated when it differs from this entity's canonical label.
3DISCO tissue clearing method
→
relatedTo
→
3DISCO tissue clearing method
self-linksurface differs
ⓘ
this entity surface form:
uDISCO tissue clearing method
3DISCO tissue clearing method
→
relatedTo
→
3DISCO tissue clearing method
self-linksurface differs
ⓘ
this entity surface form:
iDISCO tissue clearing method