CRISPR-Cas9 gene-editing technology
E366097
CRISPR-Cas9 gene-editing technology is a revolutionary molecular tool that allows scientists to precisely modify DNA within living organisms, transforming research, medicine, and biotechnology.
All labels observed (7)
| Label | Occurrences |
|---|---|
| CRISPR | 1 |
| CRISPR-Cas9 | 1 |
| CRISPR-Cas9 gene-editing method | 1 |
| CRISPR-Cas9 gene-editing technology canonical | 1 |
| CRISPR-Cas9 genome-editing method | 1 |
| SpCas9 | 1 |
| nickase Cas9 | 1 |
How this entity was disambiguated
This entity first appeared as the object of triple T3520302 — resolving that mention is where its identity was fixed. The disambiguator weighed these candidate entities and picked the highlighted one (or “None”, minting a new entity). This is how homonymy is resolved: the same surface form can point to different entities.
NED1
Entity disambiguation (via context triple)
gpt-5-mini-2025-08-07
Target entity: CRISPR-Cas9 gene-editing technology Context triple: [Emmanuelle Charpentier, knownFor, CRISPR-Cas9 gene-editing technology]
-
A.
Yamanaka factors
Yamanaka factors are a set of transcription factors that can reprogram adult somatic cells into induced pluripotent stem cells, revolutionizing regenerative medicine and stem cell research.
-
B.
Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc
Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc are a set of transcription factors whose forced expression can reprogram differentiated somatic cells into induced pluripotent stem cells.
-
C.
CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
-
D.
From Bacteria to Bach and Back
From Bacteria to Bach and Back is a philosophical work by Daniel Dennett that explores how mind, consciousness, and culture could have evolved from simple biological beginnings through natural selection and cultural evolution.
-
E.
The Language of Life
The Language of Life is a popular science book by geneticist Francis Collins that explains how advances in genomics are transforming medicine and personal health.
- F. None of above. chosen
- G. Unsure - the case is ambiguous/there is not enough information to decide.
NED2
Entity disambiguation (via description)
gpt-5-mini-2025-08-07
Target entity: CRISPR-Cas9 gene-editing technology Target entity description: CRISPR-Cas9 gene-editing technology is a revolutionary molecular tool that allows scientists to precisely modify DNA within living organisms, transforming research, medicine, and biotechnology.
-
A.
Yamanaka factors
Yamanaka factors are a set of transcription factors that can reprogram adult somatic cells into induced pluripotent stem cells, revolutionizing regenerative medicine and stem cell research.
-
B.
Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc
Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc are a set of transcription factors whose forced expression can reprogram differentiated somatic cells into induced pluripotent stem cells.
-
C.
CLARITY tissue-clearing method
CLARITY is a neuroscience technique that renders biological tissues transparent while preserving their molecular structure, enabling high-resolution, three-dimensional imaging of intact organs such as the brain.
-
D.
From Bacteria to Bach and Back
From Bacteria to Bach and Back is a philosophical work by Daniel Dennett that explores how mind, consciousness, and culture could have evolved from simple biological beginnings through natural selection and cultural evolution.
-
E.
The Language of Life
The Language of Life is a popular science book by geneticist Francis Collins that explains how advances in genomics are transforming medicine and personal health.
- F. None of above. chosen
Statements (59)
| Predicate | Object |
|---|---|
| instanceOf |
biotechnology
ⓘ
genetic engineering tool ⓘ genome editing technology ⓘ molecular biology technique ⓘ |
| appliedIn |
agriculture
ⓘ
basic biological research ⓘ biotechnology ⓘ industrial microbiology ⓘ medicine ⓘ |
| associatedWithScientist |
Emmanuelle Charpentier
ⓘ
Feng Zhang ⓘ Jennifer Doudna ⓘ |
| basedOn | CRISPR adaptive immune system in bacteria ⓘ |
| canBeDeliveredBy |
lipid nanoparticles
ⓘ
plasmids ⓘ ribonucleoprotein complexes ⓘ viral vectors ⓘ |
| characterizedBy |
high efficiency
ⓘ
programmable DNA targeting ⓘ relative simplicity ⓘ |
| developedFrom | Streptococcus pyogenes CRISPR system ⓘ |
| enables |
functional genomics studies
ⓘ
gene correction ⓘ gene knock-in ⓘ gene knockout ⓘ precise genome modification ⓘ regulation of gene expression ⓘ targeted DNA double-strand breaks ⓘ |
| firstDemonstratedIn | 2012 ⓘ |
| hasMechanism |
PAM sequence recognition
ⓘ
RNA-guided DNA cleavage ⓘ |
| hasVariant |
Cpf1 (Cas12a)
ⓘ
SaCas9 ⓘ CRISPR-Cas9 gene-editing technology self-linksurface differs ⓘ
surface form:
SpCas9
dead Cas9 (dCas9) ⓘ high-fidelity Cas9 ⓘ CRISPR-Cas9 gene-editing technology self-linksurface differs ⓘ
surface form:
nickase Cas9
|
| raisesIssue |
ethical concerns
ⓘ
germline editing debates ⓘ mosaicism ⓘ off-target effects ⓘ |
| recognizedBy | 2020 Nobel Prize in Chemistry ⓘ |
| regulatedBy | national biosafety authorities ⓘ |
| requires | protospacer adjacent motif ⓘ |
| subjectOf | patent disputes ⓘ |
| transformed |
functional genomics research
ⓘ
genetic engineering practices ⓘ |
| typicallyUsesPAM | NGG sequence for SpCas9 ⓘ |
| usedFor |
antiviral research
ⓘ
cancer research ⓘ creating genetically modified organisms ⓘ crop trait improvement ⓘ developing gene therapies ⓘ disease modeling in animals ⓘ engineering immune cells ⓘ high-throughput genetic screens ⓘ pathogen resistance in plants ⓘ |
| usesComponent |
Cas9 nuclease
ⓘ
guide RNA ⓘ |
How these facts were elicited
The pipeline generated the facts above by prompting gpt-5.1 with this entity's name + description and the instruction below.
Instruction
You are a knowledge base construction expert. Given a subject entity and a description of it, return factual statements that you know for the subject as a JSON list of dictionaries(triples), where keys must be "subject", "predicate" and "object". The number of facts may be very high, between 25 to 50 or more, for very popular subjects. For less popular subjects, the number of facts can be very low, like 5 or 10. # Requirements - If you don't know the subject at all, return an empty list. - If the subject is not a named entity, return an empty list. - Include at least one triple where predicate is "instanceOf". - Do not get too wordy. - Separate several objects into multiple triples with one object.
Input
Subject: CRISPR-Cas9 gene-editing technology Description of subject: CRISPR-Cas9 gene-editing technology is a revolutionary molecular tool that allows scientists to precisely modify DNA within living organisms, transforming research, medicine, and biotechnology.
Referenced by (7)
Full triples — surface form annotated when it differs from this entity's canonical label.
this entity surface form:
CRISPR-Cas9 gene-editing method
this entity surface form:
CRISPR-Cas9 genome-editing method
this entity surface form:
CRISPR-Cas9
CRISPR-Cas9 gene-editing technology
→
hasVariant
→
CRISPR-Cas9 gene-editing technology
self-linksurface differs
ⓘ
this entity surface form:
SpCas9
CRISPR-Cas9 gene-editing technology
→
hasVariant
→
CRISPR-Cas9 gene-editing technology
self-linksurface differs
ⓘ
this entity surface form:
nickase Cas9
A Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution
→
mainSubject
→
CRISPR-Cas9 gene-editing technology
ⓘ
this entity surface form:
CRISPR