Lowenstein–Jensen medium

E271796

Lowenstein–Jensen medium is a specialized egg-based solid growth medium used primarily for the isolation and cultivation of Mycobacterium species, especially Mycobacterium tuberculosis, in clinical microbiology.

All labels observed (1)

Label Occurrences
Lowenstein–Jensen medium canonical 2

How this entity was disambiguated

Statements (49)

Predicate Object
instanceOf culture medium
egg-based medium
selective medium
solid growth medium
alternativeTo Middlebrook 7H10 agar
Middlebrook 7H10 agar
surface form: Middlebrook 7H11 agar
classifiedAs biosafety level 3 culture medium when used with M. tuberculosis
colonyMorphologyOnMedium rough, buff-colored colonies of Mycobacterium tuberculosis
developedBy Ernst Lowenstein
Kurt Jensen
glycerolEffect enhances growth of Mycobacterium tuberculosis
hasComponent asparagine
disodium phosphate
glycerol
magnesium sulfate
malachite green
mineral salts
monopotassium phosphate
potato flour or starch
whole eggs
hasPreparationStep coagulation by inspissation
dispensing into tubes or bottles
mixing of mineral salt solution with eggs and other components
hasProperty green color due to malachite green
inhibits most contaminating bacteria
selective for mycobacteria
solidified by coagulation of eggs
hasVariant Lowenstein–Jensen medium with pyruvate
Lowenstein–Jensen medium without glycerol
inhibitsGrowthOf most Gram-negative bacteria
most Gram-positive bacteria
pyruvateEffect enhances growth of Mycobacterium bovis
qualityControlOrganism Mycobacterium tuberculosis
surface form: Mycobacterium tuberculosis H37Rv strain
requiresIncubationAtmosphere aerobic conditions
requiresIncubationTemperature 35–37 °C
storageCondition refrigerated before use
supportsGrowthOf Mycobacterium tuberculosis complex
nontuberculous mycobacteria
typicalIncubationTime up to 8 weeks for visible mycobacterial colonies
usedFor cultivation of Mycobacterium species
cultivation of Mycobacterium tuberculosis
isolation of Mycobacterium species
isolation of Mycobacterium tuberculosis
usedIn clinical microbiology
diagnosis of tuberculosis
usedWithSpecimenType body fluids suspected of mycobacterial infection
bronchoalveolar lavage
sputum
tissue biopsies

How these facts were elicited

Referenced by (2)

Full triples — surface form annotated when it differs from this entity's canonical label.

Mycobacterium tuberculosis cultureMedium Lowenstein–Jensen medium
Middlebrook 7H10 agar comparedWith Lowenstein–Jensen medium