phiC31 integrase system
E459330
The phiC31 integrase system is a site-specific recombination tool widely used in genetic engineering to insert DNA precisely into defined genomic locations.
Statements (47)
| Predicate | Object |
|---|---|
| instanceOf |
genetic engineering tool
ⓘ
site-specific recombination system ⓘ |
| derivedFrom | Streptomyces phage phiC31 NERFINISHED ⓘ |
| developedFor | precise, predictable transgene insertion compared to random integration methods ⓘ |
| hasApplication |
chromosomal docking site strategies
ⓘ
functional genomics studies ⓘ gene therapy vector integration ⓘ generation of stable cell lines ⓘ genome engineering research ⓘ germline transgenesis in Drosophila melanogaster ⓘ germline transgenesis in mice ⓘ germline transgenesis in mosquitoes ⓘ germline transgenesis in rats ⓘ germline transgenesis in zebrafish ⓘ insertion of reporter constructs into defined loci ⓘ safe harbor locus targeting ⓘ site-specific integration in mammalian genomes ⓘ transgenesis in human cell lines ⓘ transgenesis in livestock ⓘ transgenesis in plants ⓘ |
| hasComponent |
attB recognition site
ⓘ
attP recognition site ⓘ phiC31 integrase enzyme ⓘ |
| hasEngineeringVariant |
codon-optimized phiC31 integrase for mammalian expression
ⓘ
mutant integrase variants with altered specificity ⓘ nuclear localization signal-tagged phiC31 integrase ⓘ |
| hasFunction |
enables precise DNA integration into defined genomic loci
ⓘ
facilitates stable genomic insertion of transgenes ⓘ mediates unidirectional site-specific recombination between attP and attB sites ⓘ |
| hasLimitation |
irreversibility of integration without recombination directionality factors
ⓘ
potential off-target integration at pseudo-attP or pseudo-attB sites in eukaryotic genomes ⓘ |
| hasProperty |
does not require host recombination factors
ⓘ
functions in a wide range of species ⓘ generates attL and attR hybrid sites after recombination ⓘ recognizes specific attB and attP sequences ⓘ unidirectional under normal conditions ⓘ |
| mechanism |
integrase binds attP and attB sites and catalyzes recombination
ⓘ
recombination produces attL and attR sites that are refractory to further integration ⓘ |
| requiresCofactor | divalent metal ions ⓘ |
| usedIn |
Drosophila attP docking site transgenic platforms
ⓘ
induced pluripotent stem cell engineering ⓘ mammalian safe harbor loci such as ROSA26 ⓘ stable integration of therapeutic genes in preclinical models ⓘ |
| usedWith |
expression cassettes for transgenes
ⓘ
genomic docking sites containing attP sequences ⓘ plasmid vectors carrying attB sites ⓘ |
| usesRecombinationType | serine recombinase-mediated recombination ⓘ |
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.