Middlebrook 7H9 broth

E271798

Middlebrook 7H9 broth is a nutrient-rich liquid growth medium commonly used for the laboratory cultivation and study of mycobacteria, including Mycobacterium tuberculosis.

All labels observed (1)

Label Occurrences
Middlebrook 7H9 broth canonical 1

How this entity was disambiguated

Statements (49)

Predicate Object
instanceOf liquid culture medium
microbiological growth medium
mycobacterial culture medium
aggregateState liquid
commerciallyAvailableFrom BD Biosciences
Difco
various microbiological media suppliers
containsComponent L-glutamic acid
ammonium sulfate
beef extract
ferric ammonium citrate
glycerol
magnesium sulfate
pancreatic digest of casein
potassium phosphate dibasic
potassium phosphate monobasic
sodium citrate
cultureMediumFor Mycobacterium bovis
surface form: Mycobacterium bovis BCG

Mycobacterium tuberculosis
non-tuberculous mycobacteria
developedBy George Middlebrook
hasForm liquid
hasPHRange approximately 6.6 to 7.0
hasProperty nutrient-rich
supports dispersed mycobacterial growth with surfactant
supports slow-growing bacteria
namedAfter George Middlebrook
relatedTo Middlebrook 7H10 agar
Middlebrook 7H11 agar
supplementedWith OADC
Tween 80
glycerol
oleic acid albumin dextrose catalase supplement
typicalIncubationAtmosphere aerobic
typicalIncubationTemperature 37 °C
usedFor antimicrobial susceptibility testing of mycobacteria
cultivation of Mycobacterium tuberculosis
cultivation of mycobacteria
drug screening against Mycobacterium tuberculosis
growth of fast-growing mycobacteria
growth of slow-growing mycobacteria
maintenance of mycobacterial laboratory strains
mycobacterial growth curve studies
preparation of mycobacterial inocula
usedIn clinical mycobacteriology laboratories
drug discovery for tuberculosis
minimum inhibitory concentration assays for mycobacteria
tuberculosis research
usedWith shaking incubation for mycobacterial cultures

How these facts were elicited

Referenced by (1)

Full triples — surface form annotated when it differs from this entity's canonical label.

Mycobacterium tuberculosis cultureMedium Middlebrook 7H9 broth