Kinyoun stain
E929611
Kinyoun stain is a cold acid-fast staining technique used in microbiology as a safer, non-heated alternative to the Ziehl–Neelsen method for detecting organisms like Mycobacterium species.
Statements (47)
| Predicate | Object |
|---|---|
| instanceOf |
acid-fast staining technique
ⓘ
laboratory technique ⓘ microbiological staining method ⓘ |
| advantage |
avoids production of potentially infectious aerosols from heating
ⓘ
safer than heated Ziehl–Neelsen method ⓘ simpler equipment requirements ⓘ |
| alsoKnownAs |
Kinyoun method
NERFINISHED
ⓘ
cold acid-fast stain ⓘ |
| appliedTo |
clinical specimens
ⓘ
sputum smears ⓘ tissue sections ⓘ |
| basedOn | Ziehl–Neelsen stain NERFINISHED ⓘ |
| comparedTo | Ziehl–Neelsen stain NERFINISHED ⓘ |
| developedBy | Joseph J. Kinyoun NERFINISHED ⓘ |
| field |
medical microbiology
ⓘ
microbiology ⓘ |
| hasCharacteristic |
carbol fuchsin primary stain
ⓘ
cold method ⓘ does not require heating to drive stain into cells ⓘ high concentration of basic fuchsin ⓘ high concentration of phenol ⓘ non-heated method ⓘ retains primary stain in acid-fast organisms after decolorization ⓘ |
| relatedTo |
Auramine–rhodamine stain
ⓘ
Ziehl–Neelsen stain NERFINISHED ⓘ fluorochrome acid-fast stain ⓘ |
| requiresStep |
application of primary stain
ⓘ
counterstaining ⓘ decolorization with acid-alcohol ⓘ |
| safetyConsideration |
avoids open flame heating of slides
ⓘ
phenol is toxic and requires ventilation ⓘ |
| usedFor |
detection of Mycobacterium species
ⓘ
detection of Mycobacterium tuberculosis ⓘ detection of Nocardia species ⓘ detection of acid-fast bacteria ⓘ detection of partially acid-fast organisms ⓘ |
| usedIn |
clinical microbiology laboratories
ⓘ
diagnosis of mycobacterial infections ⓘ diagnosis of tuberculosis ⓘ |
| usesReagent |
acid-alcohol decolorizer
ⓘ
brilliant green counterstain ⓘ carbol fuchsin ⓘ methylene blue counterstain ⓘ phenol ⓘ sulfuric acid decolorizer ⓘ |
| visualizes |
blue background with methylene blue counterstain
ⓘ
red acid-fast bacilli ⓘ |
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.