ZN stain
E928060
ZN stain is a microbiological staining technique used to detect acid-fast organisms, particularly Mycobacterium species such as the tuberculosis bacillus.
Statements (47)
| Predicate | Object |
|---|---|
| instanceOf |
acid-fast staining method
ⓘ
differential staining method ⓘ microbiological staining technique ⓘ |
| advantage | high specificity for acid-fast bacilli ⓘ |
| alternativeName | acid-fast bacilli stain ⓘ |
| appliedTo |
clinical samples suspected of mycobacterial infection
ⓘ
sputum smears ⓘ tissue sections ⓘ |
| biosafetyConsideration | requires handling of potentially infectious aerosols ⓘ |
| category | hot acid-fast staining method ⓘ |
| colorOfBackground | blue or green depending on counterstain ⓘ |
| colorOfOrganisms | bright red rods ⓘ |
| comparedWith | Auramine–rhodamine fluorescent stain ⓘ |
| detects |
Mycobacterium leprae
NERFINISHED
ⓘ
Nocardia species (partially acid-fast) NERFINISHED ⓘ other acid-fast bacteria ⓘ |
| fieldOfUse |
clinical microbiology
ⓘ
histopathology ⓘ infectious disease diagnostics ⓘ |
| fullName | Ziehl–Neelsen stain NERFINISHED ⓘ |
| hasVariant |
Kinyoun stain
NERFINISHED
ⓘ
cold Ziehl–Neelsen method NERFINISHED ⓘ |
| limitation | lower sensitivity than some fluorescent staining methods ⓘ |
| microscopyType | light microscopy ⓘ |
| namedAfter |
Franz Ziehl
NERFINISHED
ⓘ
Friedrich Neelsen NERFINISHED ⓘ |
| observationMagnification | oil immersion microscopy ⓘ |
| principle |
acid-fast organisms retain primary stain despite acid-alcohol decolorization
ⓘ
high mycolic acid content in cell wall confers acid-fastness ⓘ |
| samplePreparation | smear fixation by heat ⓘ |
| stainingTarget | mycolic acid–rich cell walls ⓘ |
| step |
application of carbol fuchsin with heating
ⓘ
counterstaining with methylene blue or malachite green ⓘ decolorization with acid-alcohol ⓘ |
| usedFor |
demonstration of acid-fast bacilli in clinical specimens
ⓘ
detection of Mycobacterium species ⓘ detection of Mycobacterium tuberculosis ⓘ detection of acid-fast organisms ⓘ diagnosis of tuberculosis ⓘ |
| usedIn | resource-limited settings for TB diagnosis ⓘ |
| usesCounterstain |
malachite green
ⓘ
methylene blue ⓘ |
| usesDecolorizer | acid-alcohol ⓘ |
| usesMordantOrAid | heat ⓘ |
| usesPrimaryStain | carbol fuchsin ⓘ |
| visualResult |
acid-fast bacilli appear red
ⓘ
non–acid-fast elements appear blue or green depending on counterstain ⓘ |
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.