green fluorescent protein
E854252
Green fluorescent protein (GFP) is a naturally fluorescent protein originally found in jellyfish that is widely used as a marker in molecular and cell biology to visualize gene expression and protein localization.
Observed surface forms (1)
| Surface form | Occurrences |
|---|---|
| Green fluorescent protein | 0 |
Statements (52)
| Predicate | Object |
|---|---|
| instanceOf |
biological marker
ⓘ
fluorescent protein ⓘ protein ⓘ reporter gene ⓘ |
| discoveredInTaxon | Aequorea victoria NERFINISHED ⓘ |
| emitsFluorescenceColor | green ⓘ |
| fluorescenceIs | autocatalytic ⓘ |
| formsStructure | beta-barrel ⓘ |
| hasAminoAcidLength | 238 amino acids ⓘ |
| hasApplicationIn |
cell biology
ⓘ
developmental biology ⓘ molecular biology ⓘ neuroscience ⓘ |
| hasChromophore | Ser65-Tyr66-Gly67 tripeptide ⓘ |
| hasCommonAbbreviation | GFP ⓘ |
| hasEmissionWavelength | 509 nm ⓘ |
| hasExcitationWavelength |
395 nm
ⓘ
475 nm ⓘ |
| hasMolecularWeight | about 27 kDa ⓘ |
| hasMutant |
EGFP
ⓘ
blue fluorescent protein ⓘ cyan fluorescent protein ⓘ enhanced GFP NERFINISHED ⓘ yellow fluorescent protein ⓘ |
| hasProperty |
forms stable beta-barrel scaffold
ⓘ
genetically encodable ⓘ intrinsically fluorescent ⓘ non-invasive reporter ⓘ |
| isEncodedBy | gfp gene ⓘ |
| isExcitedBy |
blue light
ⓘ
ultraviolet light ⓘ |
| isExpressedIn |
bacteria in recombinant form
ⓘ
mammalian cells in recombinant form ⓘ transgenic animals ⓘ yeast in recombinant form ⓘ |
| isUsedAs |
fusion tag
ⓘ
reporter protein ⓘ |
| isUsedFor |
biosensor construction
ⓘ
cell lineage tracing ⓘ live-cell imaging ⓘ promoter activity assays ⓘ protein localization studies ⓘ protein–protein interaction studies ⓘ visualizing gene expression ⓘ |
| isVisualizedBy | fluorescence microscopy ⓘ |
| NobelPrize2008LaureatesInclude |
Martin Chalfie
NERFINISHED
ⓘ
Osamu Shimomura NERFINISHED ⓘ Roger Y. Tsien NERFINISHED ⓘ |
| originallyIsolatedFrom | Aequorea victoria GENERATED ⓘ |
| requires | molecular oxygen for chromophore maturation ⓘ |
| requiresCofactor | none external ⓘ |
| wasCoAwardedNobelPrizeIn | Chemistry 2008 NERFINISHED ⓘ |
Referenced by (1)
Full triples — surface form annotated when it differs from this entity's canonical label.