Benedict's qualitative reagent for glucose

E470215

Benedict's qualitative reagent for glucose is a chemical solution used in clinical and laboratory settings to detect and estimate reducing sugars, particularly glucose, through a characteristic color change upon heating.

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Statements (47)

Predicate Object
instanceOf chemical reagent
diagnostic reagent
qualitative analytical reagent
alsoKnownAs Benedict's reagent NERFINISHED
appearance blue solution
brickRedPrecipitateIndicates high concentration of reducing sugar
carbonateRole provides alkaline medium
citrateRole complexes copper(II) ions
colorChange blue to brick-red
blue to green
blue to orange
blue to yellow
colorIndicates approximate concentration of reducing sugar
containsIon Cu2+
detects fructose
glucose
lactose
maltose
reducing sugars
doesNotDetect most non-reducing sugars without prior hydrolysis
forms cuprous oxide precipitate
greenColorIndicates low concentration of reducing sugar
hasComponent copper(II) sulfate pentahydrate
sodium carbonate
sodium citrate
hazard alkaline irritant
contains copper salts
mechanism reduction of Cu2+ to Cu+
namedAfter Stanley Rossiter Benedict NERFINISHED
pH alkaline
reactionType redox reaction
replacedBy enzymatic glucose assays in many clinical laboratories
requires heating with sample
sampleType biological fluids
urine
solvent water
stabilizedBy sodium citrate
storageCondition protected from contamination
tightly stoppered bottle
usedFor detection of glucose
detection of reducing sugars
semi-quantitative estimation of reducing sugars
usedIn biochemistry laboratories
clinical laboratories
educational laboratories
usedInTest Benedict's test NERFINISHED
yellowToOrangeColorIndicates moderate concentration of reducing sugar

Referenced by (1)

Full triples — surface form annotated when it differs from this entity's canonical label.

Stanley Rossiter Benedict notableWork Benedict's qualitative reagent for glucose